Cell Support Services

cell support services

NovoHelix offers a range of cell services to ensure success of custom ‘disease-in-a-dish’ cell models. These service requests include gamma-irradiation of feeder cells, embryonic fibroblast derivation for co-culture of pluripotent stem cells, primary cell immortalization, and establishment of primary cell lines from elite animals for somatic cell nuclear transfer (SCNT) and genome engineering. We offer flexible modalities including adherent and suspension bioreactors for scalable culture, single-cell cloning and expansion, and colony screening in 96-well or 384-well microplate formats. Although we have not enumerated ever cell service that we offer, please inquire through our online quote portal for requests that are not directly listed.

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services

Service

Catalog Nr

Service Description

Price

cell line gamma-irradiation

CMSS001

NovoHelix offers gamma-irradiation service for irradiating cell lines (patient-derived, pooled, genome edited/ corrected cell lines) using a gamma-emission source to irradiate cells at a constant dose rate and to any desired extent of radiation. Irradiated cell line models are novel toolkits for applications such as generation of co-culture models using non-dividing/ feeder cells; to study mechanisms underlying cancer progression; wound healing and inflammatory responses after irradiation; screen drugs for enhancing irradiation sensitivity of tumor cells; and for xenograft tumor models.

Our Cell Line Irradiation Service includes:

Cell line expansion/ harvesting

Cell line Irradiation (customized extent of irradiation)

Cryopreservation

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cell immortalization

CMSS002

NovoHelix offers patient fibroblast immortalization service via the simian virus 40 T antigen.

Simian virus 40 (SV40) T antigen has been shown to be the simplest and most reliable agent for the immortalization of many different cell types and the mechanism of SV40 T antigen in cell immortalization is relatively well understood. Recent studies have also shown that SV40 T antigen can induce telomerase activity in the infected cells. We have experience with human, mouse, pig fibroblasts immortalization by introducing SV40 T antigen.

Service includes:

Lentivirus vector(s) expressing SV40 large and small T antigens

Transduction

colony selection (up to 10 passages)

Characterization (transgene expression by RT-PCR)

Culture, cryopreservation

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primary cell isolation

CMSS003

NovoHelix can isolate primary cells from fresh tissues, including from biopsy samples.

Primary cells differ from immortalized cell lines because they have a limited population doubling time. However, primary cells retain the physiological properties of the tissue of origin and mimic in vivo conditions. Primary cells are ideal cell models for a variety of research, including cell biology/physiology, disease mechanisms, drug screening and therapeutic development. NovoHelix can isolate a specific cell type from tissues including (but not limited to): Skin, Heart, Liver, Kidney, Bone, Skeletal Muscle, Brain or other nervous tissue.

We first isolate the cell-type-of interest using magnetic bead technology. Cells are then frozen in liquid nitrogen for long-term storage. Thawed cells are subject to flow cytometry and plated for immunocytochemistry for further characterization. We also provide recommended growth and handling conditions for you with each purchase.

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references

Garfield AS. Derivation of primary mouse embryonic fibroblast (PMEF) cultures. Methods Mol Biol. 2010;633:19-27. doi: 10.1007/978-1-59745-019-5_2. PubMed PMID: 20204617.

Unger C, Felldin U, Nordenskjöld A, Dilber MS, Hovatta O. Derivation of human skin fibroblast lines for feeder cells of human embryonic stem cells. Curr Protoc Stem Cell Biol. 2008 Jun;Chapter 1:Unit 1C.7. doi: 10.1002/9780470151808.sc01c07s5. PubMed PMID: 18770630.

Meissner A, Eminli S, Jaenisch R. Derivation and manipulation of murine embryonic stem cells. Methods Mol Biol. 2009;482:3-19. doi: 10.1007/978-1-59745-060-7_1. PubMed PMID: 19089346.

E Michalska A. Isolation and propagation of mouse embryonic fibroblasts and preparation of mouse embryonic feeder layer cells. Curr Protoc Stem Cell Biol. 2007 Oct;Chapter 1:Unit1C.3. doi: 10.1002/9780470151808.sc01c03s3. PubMed PMID: 18785164.

Lin JY, Simmons DT. The ability of large T antigen to complex with p53 is necessary for the increased life span and partial transformation of human cells by simian virus 40. J Virol. 1991 Dec;65(12):6447-53. PubMed PMID: 1658353; PubMed Central PMCID: PMC250682.

Jaskelioff M, Muller FL, Paik JH, Thomas E, Jiang S, Adams AC, Sahin E, Kost-Alimova M, Protopopov A, Cadiñanos J, Horner JW, Maratos-Flier E, Depinho RA. Telomerase reactivation reverses tissue degeneration in aged telomerase-deficient mice. Nature. 2011 Jan 6;469(7328):102-6. doi: 10.1038/nature09603. Epub 2010 Nov 28. PubMed PMID: 21113150; PubMed Central PMCID: PMC3057569.

Sun L, Chiang JY, Choi JY, Xiong ZM, Mao X, Collins FS, Hodes RJ, Cao K. Transient induction of telomerase expression mediates senescence and reduces tumorigenesis in primary fibroblasts. Proc Natl Acad Sci U S A. 2019 Sep 17;116(38):18983-18993. doi: 10.1073/pnas.1907199116. Epub 2019 Sep 3. PubMed PMID: 31481614; PubMed Central PMCID: PMC6754593.

Meissner A, Jaenisch R. Mammalian nuclear transfer. Dev Dyn. 2006 Sep;235(9):2460-9. Review. PubMed PMID: 16881069.

Wilmut I, Bai Y, Taylor J. Somatic cell nuclear transfer: origins, the present position and future opportunities. Philos Trans R Soc Lond B Biol Sci. 2015 Oct 19;370(1680):20140366. doi: 10.1098/rstb.2014.0366. Review. PubMed PMID: 26416677; PubMed Central PMCID: PMC4633995.

Keefer CL. Artificial cloning of domestic animals. Proc Natl Acad Sci U S A. 2015 Jul 21;112(29):8874-8. doi: 10.1073/pnas.1501718112. Review. PubMed PMID: 26195770; PubMed Central PMCID: PMC4517265.

Li R, Miao J, Wang Z. Production of Genetically Engineered Porcine Embryos by Handmade Cloning. Methods Mol Biol. 2019;1874:347-360. doi: 10.1007/978-1-4939-8831-0_20. PubMed PMID: 30353524.