Transgene design, construct generation, transgene purification and, pronuclear microinjection

Knockins (KI): site-specific point mutations, protein tag insertions, reporter gene knockins, safer harbor site knockins, whole gene replacement (humanization)

Recombineering-modified BACs (bacterial artificial chromosomes) via pronuclear microinjection

Tissue-specific or inducible knockouts that bypass embryonic lethality from C57BL/6, BALB/c or 129P2/Ola strains

Frameshift stop (indels), exon deletions, single or multi-gene deletions, double knockouts (DKO), deletion of linked genes