Robust, transferable genotyping assays are needed to screen many of the animal and cellular models during our production and validation cycles. NovoHelix can develop simple and robust genotyping protocols for all types of models in our portfolio including CRISPR models. One of the core skills of the laboratory is the systematic collection of specimens for DNA/RNA extraction, and our team can isolate nucleic acid from skin, toes, tail, and many other tissues, organs, cell pellets, and embryos. We have experience genotyping various animal models including mice, rats, pigs, cows, goats, and multiple human pathology tissues and human cell lines.
NovoHelix also provides expert assay design and consultation to the client to ensure genotyping success. Assay design costs are based on the number of alleles screened. In some cases, a synthetic positive control needs to be built for assay validation and these 'construction' costs will be passed on to the client.
In addition to sample collection and genotyping, we provide pathogen detection services for mycoplasma and adventitious viruses. We offer cell line expansion and cryopreservation and can authenticate these cell line resources to enhance reproducibility. Indeed, NovoHelix has developed STR profiling and SNP assays to obviate cell line misidentification and contamination as mandated prior to publication in high-impact journals and encouraged by investigators funded by the NIH. A detailed cost estimate can be developed based on your specific project through our online Quote Request.
Pathogen + Mycoplasma testing of client-provided ES cell lines/cell lines
A panel of over 20 viral pathogens are screened including coronavirus, reovirus and Sendai virus. This service also includes mycoplasma testing.
Mycoplasma testing of ES cell clones/cell lines provided by the client
NovoHelix provides mycoplasma testing using a multiplex 16S rRNA gene assay to broadly determine adventitious mycoplasma species infection. This service fee is per mouse ES cell (mESC) clone/cell line. The assay detects over 60 species of Mycoplasma, Acholeplasma, Spiroplasma and Ureaplasma including the eight species most likely to afflict cell cultures: M. arginini, M. fermentans, M. hominis, M.hyorhinis, M. orale, M. pirum, M. salivarium, and A. laidlawii.
Genotyping Assay Development
Robust, transferable genotyping assays are needed to screen many of the animal and cellular models during our production and validation cycles. NovoHelix provides expert assay design and consultation to the client to ensure genotyping success. Assay design costs are based on the number of alleles screened. In some cases, a synthetic positive control needs to be built for assay validation and these 'building' costs will be passed on to the client.
Genotyping includes DNA extraction and purification services with a minimum charge of 10 samples. Samples that require additional genotyping validation such as Sanger sequencing of frameshifting mutants or point mutations will incur additional fee schedules (sequencing costs, etc). Genotyping is $15 per sample per allele screened.
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