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reproductive services
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reproductive services

NovoHelix offers a variety of reproductive services to help clients protect valuable mouse strains from genetic drift and reproductive obstacles. Contact us for pricing and options regarding the services listed below:

•strain rederivation for pathogen-free mouse lines produced in our barrier facility
•cryopreservation of mouse sperm and embryos
•recovery of cryopreserved mouse sperm and embryos
•in vitro fertilization (IVF)
•intracytoplasmic sperm injection (ICSI)
•ovarian transplantation
•tetraploid embryo complementation



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reproductive service

Service

Catalog Nr

Service Description

Price

 Mouse ovary transplantation
REPS001

 Ovary transplantation is used on a genetically valuable female such as a CRISPR-edited allele, transgenic allele, novel allele; that is not transmitting due to health complications. Ovary transplantation is an advanced reproductive technique, and NovoHelix does not provide a guarantee that the recipient will transmit the desired allele.  Session includes sedation, transfer into the bursal membrane of recipient, and breeding for F1's. Ovary transplants will be transferred into a minumum of two recipients.  Sessions include ovaries from one donor.  Additional sessions may be purchased to ensure highest probabilty of transfer if multiple donors are available.

 Mouse Animal Husbandry
REPS002

 mouse colony management including breeding, weaning, sampling, monitoring, per cage per day 

Rat Animal Husbandry
 REPS019
rat colony management including breeding, weaning, sampling, monitoring, per cage per day 
Pig Animal Husbandry
REPS020

pig management including breeding, weaning, sampling, monitoring, per cage per day 

Goat Animal Husbandry
 REPS021
goat management including breeding, weaning, sampling, monitoring, per stall per day 
Horse Animal Husbandry
REPS022

horse stall management including breeding, weaning, sampling, monitoring, per stall per day 

Cow Animal Husbandry
 REPS023
cow management including breeding, weaning, sampling, monitoring, per stall per day 
Hamster Animal Husbandry
REPS024

hamster cage management including breeding, weaning, sampling, monitoring, per cage per day 

 IVF/Sperm Cryorecovery 
 REPS003
 In vitro fertilization with client-provided sperm, standard strains


B6, FVB, SW; inquire for pricing on other strains; does not include genotyping
 ICSI
REPS004

 Piezo-actuated intra-cytoplasmic sperm injection with client-provided sperm for rescue of a mouse line includes up to 2 sessions.

 Embryo Transfer & Cryorecovery 
REPS005
 Recovery of cryopreserved embryos provided by the client and transfer of embryos into pseudopregnant recipients includes up to 2 sessions 
 Strain Rederivation
REPS006

 Rederivation of a mouse line from fresh sperm into a clean barrier facility by IVF. Client should provide 5 males (8-20 weeks old).

 Embryo Cryopreservation + Embryo Culture QC 
REPS007
 Cryopreservation of mouse embryos derived from up to 2 sessions of IVF with client-provided donor females and cryoarchiving up to 300 embryos as heterozygous line.  A fraction of embryos will be thawed and cultured to blastocyst stage to determine cryorecovery rate for quality control. Mouse embryo cryopreservation requires at lease 7 fertile males and 14 females (4-6 weeks) for 5 straws (20 embryos per straw).
 Sperm Cryopreservation + IVF/Embryo Culture QC 
REPS008

 Cryopreservation of mouse sperm from 2 males (sperm donors should be provided by client).  IVF and embryo culture to the blastocyst stage will be performed to determine cryorecovery rate.  The service will provide cryopreserved semen in 10 straws.

Sperm/Embryo Cryostorage (annual charge, prorated 1st year)
REPS009
 One year prepaid of cryopreservation storage for one mouse strain (up to 20 embryo straws and/or 30 sperm vials). Straws or vials will be stored in multiple locations as a precaution.
 Sperm/Embryo Cryostorage (5 years)
REPS010

 5 years prepaid of cryopreservation storage for one mouse strain (up to 20 embryo straws and/or 30 sperm vials). Straws or vials will be stored in multiple locations as a precaution.

Importation
REPS011
 Coordinating importation of mice, germplasm, tissue, or cells from outside institutions or companies to NovoHelix
 Exportation
REPS012

 Coordination of exportation of mice, germplasm, tissue, or cells to outside institutions from NovoHelix. Additional fees will incur for liquid nitrogen, dry ice, shipping container and courier.

 Vapor Shipper Preparation and Handling
REPS013
 Liquid nitrogen refill for dry shippers and handling charges
 Contract or License Processing Fee
REPS014

 Processing business contracts, KOMP license payments, other agreements handling documents, communications and followup with campus, accounts payables, and IP agents.

Surgery
REPS015
Rodent surgery is $225 per hour and includes short-term sedation and general anesthesia.   
 Veterinarian / DVM consultation
REPS016

 Members of the veterinary team are available to support special services. Recharge request is $225/hour per DVM

Courier
REPS017
Courier or driver rates $70/hour plus current standard US mileage rates (58 cents per mile in 2019)
Microinjectionist
REPS018

 Hourly rate for special services by the microinjection technical staff

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