customized mouse models
Knockins (KI): site-specific point mutations, protein tag insertions, reporter gene knockins, safer harbor site knockins, whole gene replacement (humanization)
Recombineering-modified BACs (bacterial artificial chromosomes) via pronuclear microinjection
Inducible Cre activity with Cre-ERt2, gene swapping with recombinase-mediated cassette exchange (RMCE), flip-excision (FLEx) switches,COIN modules (conditionals by inversion) or FLIP cassettes (invertible intronic cassettes)
Frameshift stop (indels), exon deletions, single or multi-gene deletions, double knockouts (DKO), deletion of linked genes
Transmission of an allele at super-Mendelian ratios to offspring is achieved with CRISPR-Cas gene drives by an active genetic circuitry, sometimes called ‘CopyCat’ circuit cloning
Tissue-specific or inducible knockouts that bypass embryonic lethality from C57BL/6, BALB/c or 129P2/Ola strains
Design of the targeting strategy, construct generation, targeting by homologous recombination in mouse ES cells, blastocyst complementation, chimera generation