BAC recombineering, basic
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Product Details
Recombineering is a highly efficient and precise method used for the generation of seamlessly mutated bacterial artificial chromosomes (BACs) including insertion of point mutations, protein tags, landing pads and reporter genes. NovoHelix's BAC recombineering service offers counterselection approaches, inactivation of non-relevant genes on the BAC to prevent overexpression, gene replacement for humanization and BAC reduction by shaving. Our recombineering portiolo consists of successful design of complex genetic circuits, incorporation of site-specific recombinases such as Cre, CreERt2, flippase (Flp/Flpe/Flpo), Dre, VCre, Vika, Nigri & Panto, usage of heterotypic recombinase sites such as lox66/lox2272, Frt3/Frt5/Frt14/15, rox/rox12, recycling of antibiotic cassettes for selection with ampicillin (Amp), blasticidin (Bsd), carbenicillin (Carb), chloramphenicol (Cm), gentamycin (Gent), hygromycin (Hygro), kanamycin (Kan), nourseothricin (clonNAT), puromycin (Puro), spectinomycin (Spec), streptomycin (Strep), tetracycline (Tet), trimethoprim (Tmp) and zeocin (Zeo)/bleocin (Bleo)/phleomycin (Phleo). We include up to 2 recombineering steps for our basic service. Basic service includes selection with ampicillin, carbenicillin, chloramphenicol, kanamycin, spectinomycin, tetracycline or trimethoprim. Additional charges may incur for more recombineering steps and selection with antibiotics not included in our basic service. The modified BAC will be provided in the E. coli strain as a glycerol stock or stab culture.