Conditional Knockout -  CKOM001

 In comparison to direct knockouts, conditional knockouts (cKO) allow for temporal and spatial control of gene disruption. To generate a cKO, the target gene’s critical exons are often flanked with directional loxP sites termed ‘floxed’ which can be excised by Cre recombinase. Crossing a Cre-expression driver mouse to this floxed allele affords the investigator to conditionally excise the targeted gene only in cells which express the recombinase Cre. Conditional gene disruption is useful for modeling embryonic lethal genes, tissue-specific gene deletion or developmental-stage gene disruption.  The service includes donor vector or long single-stranded DNA (lssDNA) construction, guide RNA design using the latest design guidelines, i.e. full-length guides, chemically modified gRNAs, truncated sgRNAs, hp-sgRNAs (hairpin-sgRNAs), that produces high on-target activity in our surrogate cell-based gene editing assay while limiting off-target activity.