ES Cells/Gene Targeting - ESGT031

Purification of supercoiled plasmid by a proprietary anion exchange (AEX) chromatography, validation by restriction digestion and analysis by gel electrophoresis. Endotoxin levels are generally very low (0.05  – 0.5 ng LPS/µg) via our proprietary extraction method and are adequate for sensitive applications such as zygotic microinjection of mammalian embryos. The plasmid is predominantly in its supercoiled topology and free of RNA and protein contamination.  Linearization with a restriction endonuclease or with a homing nuclease such as PI-SceI and drop dialysis in nucleofection/electroporation/microinjection buffer is included in the purification service. Validation by additional restriction digestions (greater than 3) is available with commensurate fee schedule.  Purification is required to prevent excessive heat generation from arcing often due to high salt concentrations in DNA preps which can reduce the ES cell viability.