pluripotent stem cells - reprogramming - CMPR001

 Tetraploid embryo/ ES or iPSC cell aggregation includes amplification and freezing of clonal stocks, chromosome counting, mycoplasma testing, 2-cell embryo collection, electrofusion of 2-cell embryos to tetraploid embryos, overnight culture, removal of zona pellucida, aggregation with targeted ES cells (2 times per clone) or 2 iPSC clones, overnight culture, transfer of the ES cells/tetraploid aggregated blastocysts to pseudopregnant female.  A minimum of 2 chimeras are almost completely ES cell derived will be produced.  C57BL6 and 129 Svev mice will be provided for germline transmission tests.  Additional injection will be charged at $3500 per session. Note use of ES cells or iPSCs have varying potentials to produce chimeras and/or confer germline transmission.