Recombineering - MBRC003

Purification of supercoiled BACs by a proprietary anion exchange (AEX) chromatography, validation by PCR screening around the BAC at ~20-kb intervals and validation by restriction digestion and analysis by pulse-field gel electrophoresis (PFGE). Endotoxin levels are generally very low (0.05  – 0.5 ng LPS/µg) via our proprietary extraction method and are adequate for sensitive applications such as zygotic microinjection of mammalian embryos. The BAC is predominantly in its supercoiled topology and free of RNA and protein contamination.  Linearization with a homing nuclease such as PI-SceI and drop dialysis in 1x TE or microinjection buffer is included in the purification service. Validation by PCR screening at higher resolution (5-kb, 10-kb) or additional restriction digestions (greater than 3) is available with commensurate fee schedule.