Spy Cas9 NLS HiFi - P2280X
/Spy Cas9 NLS HiFi - P2280X
Spy Cas9 NLS HiFi, is an RNA-guided endonuclease that catalyzes site- specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. Spy Cas9 NLS HiFi contains a bipartite Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein. Off-target activity is dramatically reduced at the dominant off-target site while preserving approximately ~80% on-target activity relative to wildtype Cas9.
- Quantity
Product Details
size:
5 ml
305 nmol
concentration:
~10 mg/ml
62 µM